DETAILS, FICTION AND 2-FDCK HCL

The lab claims fixation will cause the dyes to leak in the cells. I believe the belief is the fact fixation may well hurt cell membranes. I did look for a paper which describes fixation employing methanol or acetic acid, following staining having a DCFDA by-product. DCFDA would be the reagent that stains ROS from the package ab113851, and I think t